Techniques for Cultivating MDA MB 231 Cells
Cells are maintained at a temperature of 37°C in an environment containing 5% CO2, and the culture medium is replaced approximately every 3 days. Additionally, it generally requires approximately 2 days for cells to recover from the process of cryopreservation. Once the cell culture reaches a confluence level of 80-85%, a subculture procedure is performed.
Could you please explain what is meant by the term "doubling time"?
The duplication period represents the duration required for a population to undergo a twofold increase in its size or value. This concept finds application in various contexts, including population expansion, inflationary trends, extraction of resources, consumption patterns of goods, compounding of interest, the expansion of cancerous growths, and numerous other instances that demonstrate growth over time.mda-mb-436 doubling time
Why should we opt for MDA-MB-231?
The MDA-MB-231 cell line, which was derived from a pleural effusion of a patient with invasive ductal carcinoma at M D Anderson, serves as a frequently utilized tool in simulating advanced-stage breast cancer. This specific cell line lacks the expression of ER, PR, and E-cadherin, and instead demonstrates the presence of mutated p53.
What is the replication period for MDA 468?
Approximately 30 to 40 hoursMDA-MB-468
Replication cycle duration: around 30 to 40 hours
Collection: harvesting of approximately 15 to 20 million cells per 80 square centimeters
Preservation: cryopreservation with a composition of 70% medium, 20% FBS, and 10% DMSO
DSMZ Scientific Information:
Mycoplasma contamination: absent in microbiological cultivation and PCR tests
Additional 21 entries
Between HER2 negative and HER2 positive, which one exhibits a more aggressive behavior?
HER2-positive breast cancer cells refer to those that exhibit elevated levels of HER2 compared to normal. While these types of cancers are prone to proliferate and disseminate more rapidly than HER2-negative breast cancers, they often exhibit a greater sensitivity to therapeutic agents that specifically target the HER2 protein.
What is the method for computing CDR?
The Calculation and Formula for the Constant Default Rate
To determine the constant default rate, one must first consider the quantity of new defaults that have occurred within a specified timeframe. This figure is then divided by the initial balance of the non-defaulted pool at the beginning of that same period. Subsequently, the result is subtracted from 1. The ultimate step involves elevating this figure to the nth power, where n corresponds to the total number of periods encompassed within a year.
Could you explain the replication rate of MDA-MB-231?
MDA-MB-231
Duplication period: approximately 25-30 hours
Cell Collection: Approximately 10-20 million cells per 80 square centimeters of surface area
Preservation Technique: cryopreserved in a mixture containing 70% medium, 20% FBS, and 10% DMSO
DSMZ Scientific Data:
Mycoplasma Status: absence confirmed through microbiological culturing and PCR testing
Additional 21 records availablevero cells
What are the defining features of MDA-MB-231?
Discussing MDA-MB-231 Cells
These particular cells exhibit a distinctive feature referred to as epithelial to mesenchymal transition (EMT). This transition is closely linked to the capacity of cancerous cells to undergo metastatic growth and infiltrate adjacent tissues. When cultured in vitro, MDA-MB-231 cells adhere securely to the surface of the culture dish and proliferate in a monolayer formation.sw1573 cell line
Could you please explain the replication cycle duration of MDA-MB-468 cells, specifically focusing on their doubling time?
The replication period ranges from approximately 30 to 40 hours, as reported by DSMZ with the identifier ACC-738, while PubMed cites a doubling time of 40.6 hours with the reference number 32576280. In contrast, NCI-DTP reports a longer period of 62 hours for MDA-MB-468. Other studies, such as those conducted by PBCF, suggest a doubling time of approximately 47 hours. Lastly, the JWGray panel recorded a replication cycle of 79.76 hours.
Is the Rule of 72 Considered as a Rough Estimation?
Determining the approximate timeframe for your money to double in value is a straightforward process. Simply utilize the figure of 72 and divide it by the desired interest rate you aim to achieve. This computation will yield the estimated number of years required for your investment to multiply in value.
